Metal-induced oxidative stress in terrestrial macrolichens

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Authors

KOVÁČIK Jozef DRESLER Sławomir PETERKOVÁ Viera BABULA Petr

Year of publication 2018
Type Article in Periodical
Magazine / Source Chemosphere
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.1016/j.chemosphere.2018.03.112
Keywords Antioxidants; Bioaccumulation; Fluorescence microscopy; Heavy metals; Oxidative stress; Reactive oxygen species (ROS)
Description Short-term (24 h) responses of Cladonia arbuscula subsp. mitis and Cladonia furcate to copper (CuII) or chromium (CrIII) excess (10 or 100 mu M) were compared. C arbuscula accumulated more Cu and Cr at higher metal doses but both species revealed depletion of K and/or Ca amount. Not only Cu but also Cr typically elevated reactive oxygen species (ROS) formation (fluorescence microscopy detection of total ROS and hydrogen peroxide) and depleted nitric oxide (NO) signal, with Cu showing more negative impact on lipid peroxidation (BODIPY 581/591 C11 staining reagent). Metals and staining reagents also affected anatomical responses and photobiont/mycobiont visibility. Principally different impact of Cu and Cr was observed at antioxidative metabolites level, indicating various ways of metal-induced ROS removal and/or metal chelation: Cu strongly depleted glutathione (GSH) and stimulated phytochelatin 2 (PC2) content while ascorbic acid accumulation was depleted by Cu and stimulated by Cr. Subsequent experiment with GSH biosynthetic inhibitor (buthionine sulfoximine, BSO) revealed that 48 h of exposure is needed to deplete GSH and BSO-induced depletion of GSH and PC2 amounts under Cu or Cr excess elevated ROS but depleted NO. These data suggest close relations between thiols, NO and appearance of oxidative stress (ROS generation) under metallic stress also in lichens. (C) 2018 Elsevier Ltd. All rights reserved.

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