A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines

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Authors

BINÓ Lucia MIKULENKOVÁ Erika ŠTĚPÁNEK Luděk BERNATÍK Ondřej VYSLOUŽIL David PEJŠKOVÁ Petra GORILÁK Peter HURANOVÁ Martina VARGA Vadimír ČAJÁNEK Lukáš

Year of publication 2022
Type Article in Periodical
Magazine / Source STAR Protocols
MU Faculty or unit

Faculty of Medicine

Citation
Web https://www.sciencedirect.com/science/article/pii/S266616672200079X
Doi http://dx.doi.org/10.1016/j.xpro.2022.101199
Keywords Cell Biology; Cell culture; Microscopy; Molecular Biology
Description Primary cilia are hair-like sensory organelles protruding from the surface of most human cells. As cilia are dynamic, several aspects of their biology can only be revealed by real-time analysis in living cells. Here we describe the generation of primary cilia reporter cell lines. Furthermore, we provide a detailed protocol of how to use the reporter cell lines for live-cell imaging microscopy analysis of primary cilia to study their growth as well as intraciliary transport. For complete details on the use and execution of this protocol, please refer to Bernatik et al. (2020) and Pejskova et al. (2020).
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