The role of sterol-binding in elicitn interaction with tobacco

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Publikace nespadá pod Fakultu sportovních studií, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
Název česky Role vazby sterolu při interakci elicitinů s tabákem
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LOCHMAN Jan KLEMPOVÁ Jitka LITERÁKOVÁ Petra PTÁČKOVÁ Nikola KAŠPAROVSKÝ Tomáš

Rok publikování 2009
Druh Článek ve sborníku
Konference FEBS Journal
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Obor Biochemie
Klíčová slova Elicitins tobacco recognition
Popis Cryptogein is a proteinaceous elicitor isolated from Phytophthora cryptogea able to trigger plant defence reaction. We prepared a series of cryptogein mutants which showed no-binding of sterol to study influence of sterol-binding for defence reaction activation. Cryptogein mutants were tested for induction of defence related genes expression covering the pathogenesis related proteins (PR1a (acidic), PR1b (basic), PR3Q) together with genes for epi-aristolochene synthase (EAS) and 9-lipooxygenase (LOX). Moreover, the accumulation of signalling compound salicylic acid (SA), phytoalexin capsidiol and malonyldialdehyde (MDA) as marker of cell death was measured. The mutants without sterol-binding activity showed different sensitivity to activate defence reaction in all tested markers; however mutant L15W/L36F demonstrated higher efficiency to trigger defence reaction. In addition, correlation of active oxygen species with EAS expression and capsidiol accumulation was observed.
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